To prevent this, the potassium hydroxide has to carefully be placed on the cotton ball and in the respirometer.
To further investigate the rate of cellular respiration in mung bean seeds, there are many future experiments that could be conducted to test the various conditions under which living organisms undergo cellular respiration.
Watch this quick rap!
Materials and Identification of Variables
By: Katie Alvarez, Kim Welch
and Chelsey Guastucci
If increasing amounts of
geminating seeds are being put
in respirometers, then the respirometer containing the most germinating seeds
will result in the highest rate of respiration because there is a greater amount of seeds undergoing cellular respiration.
Living organisms use chemical energy to perform their cellular functions. Many organisms convert the chemical energy stored in food into adenosine triphosphate (ATP), a type of chemical energy used to power a large number of cellular activities and enzymatic reactions. If oxygen is present, ATP can be generated from food molecules using aerobic cellular respiration. When oxygen is used for respiration, carbon dioxide is produced as a by-product.
In the following activities, you will investigate aerobic respiration occurring in different organisms. Keep the following equation in mind as you prepare for and work through these lab activities:
Humans breathe to facilitate aerobic cellular respiration for the purpose of ATP production. Oxygen is breathed in to create an aerobic environment and CO2 is expelled from the lungs as waste from the process of respiration. In this activity, you will investigate the effect of rest versus exercise on respiration.
Lets find out!
The purpose of this lab is to measure the rate of respiration in a living organism.
All cells need energy to live and function, so they get this energy by performing a process called cellular respiration through which they make carbon dioxide, water, and energy in the form of ATP from glucose and oxygen.
In this inquiry experiment,
different amounts of mung bean seeds (5, 10, and 15) will be put in
respirometers to measure the rate of respiration to test which
respirometer shows evidence of the most oxygen being consumed.
The Effects of Increasing Amounts of Germinating Mung Seeds on Amount of Oxygen Consumed
AP Bio Lab #6: Cellular Respiration
Hypothesis: If germinating and non germinating seeds are used, then the rate of cellular respiration for the germinating seeds will be higher than the rate of cellular respiration for the non-germinating seeds because non-germinating seeds will not be undergoing cellular respiration because they are not metabolically active.
The rate at which aerobic cellular respiration occurs can be determined by measuring ATP production, carbon dioxide production, and the amount of oxygen consumed.
Construct a graph to show the rate of respiration
One bottle of red Manometer fluid
10 Boiled mung seeds
10 Dry mung seeds
10 Germinating mung seeds
Potassium Hydroxide solution, KOH, 15%
3 Liters of room-temperature tap water
3 Capillary tubes
3 16-oz Clear Cups
Hot glue gun
Stirring rod, glass
Syringes, 5mL, 3
Click above to watch the Bozeman
Video on this lab!
Still confused about cellular respiration?
This lab relates to the topic of cellular respiration in the way that the effects of different conditions such as amount of mung bean seeds and condition of mung bean seeds were tested on the amount of oxygen consumed for cellular respiration.
An organism that is living that performs aerobic cellular respiration, such as mung bean seeds, will need to consume oxygen in order to carry out the process, which is why oxygen is essential to aerobic cellular respiration.