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Semi conservative replication of ..

Except for the chemotherapeutic toxicity, the anti-cancer effects of AS1411 included two specific mechanisms: blockade of the binding of NCL to the Bcl-2 oncogene, which induces the escape of cells from apoptosis, thereby enabling tumorigenicity, and inhibition of the signaling of the pro-survival molecule NF-κB, which otherwise maintains DNA replication and inhibits cell cycle arrest [, ].This preclinical success inspired great interest in the clinical study of AS1411.

Interference may occur during replication by the generation ofdefective interfering (DI) particles. They are formed duringinfection with various kinds of RNA viruses, such asrhabdoviruses, togaviruses, orthomyxoviruses, paramyxoviruses,coronaviruses and some DNA viruses (herpesviruses). With someviruses eg. VSV, the DI particles are smaller than regularparticles and can therefore be obtained in pure form. Theyusually contain the normal virion proteins but have a shortergenome. They are replication defective and require the helperfunctions of a normal virus co-infecting the same cell. In earlyserial passages, DI particles rapidly increase in titre, then theyield of the infectious virus, and finally the total particleyield is progressively reduced.

Bidirectional Replication of Simian Virus 40 DNA * ..

strand DNA synthesis) replication is perhaps ..

The synthesis of early proteins is the key initial step in viral DNA replication

mouse 10 Hz, 1 mT; 1-day exposure Decreased sodium ion content of liver (1982) Norton (1982) cultured 4 kHz bursts of bidirectional Increased hydroxyproline, chicken pulses with 15 Hz repetition hyaluronate, and DNA synthesis; embryo rate; 2 mT peak intensity; decreased glycosoaminoglycans; sternum four 6-h exposures during 2 increased lysozyme activity days Aarholt et al.

F. Hepadnaviruses - Hepatitis B virus employs reversetranscription for replication. The genome consists of a partiallydouble-stranded circular DNA with a complete negative strand andan incomplete positive strand. Upon entering the cell, thepositive strand is completed and transcribed. RNA transcripts arein turn reverse-transcribed into DNA by a viral enzyme in severalsteps, following closely the model of retroviruses, including ajump of the nascent positive strand from one direct repeat (DR)to another.

Explain why DNA replication is bidirectional and includes both a ..

chicken 0.5 ms bidirectional pulses Teratogenic changes in nervous system, (1983) embryo at 100 Hz (4 different circulatory system, and foregut waveforms); 0.4- to 104-µT peak intensity; 2-day exposure Archer & cultured 1 Hz, 15 - 60 mT square- Decreased collagenous and non- Ratcliffe chicken wave pulses; 7-day exposure collagenous protein synthesis; no (1983) tibia alteration in glycosoaminoglycan DNA synthesisLiboff et al.

E. Poxviruses - The striking feature of poxvirus DNA isthat the two complementary strands are joined. The replicativeintermediates, present in the cytoplasm, are special concatemerscontaining pairs of genomes connected either head to head or tailto tail.

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Multiple Choice Questions on DNA replication - MCQ …


Which of the following enzymes are used to join bits of DNA

The mode of replication is semiconservative but the nature ofthe replicative intermediates depends on the manner ofreplication. Several methods of replication can be recognized.

a) DNA ligase b) DNA polymerase c) primase d) Endonuclease 2

4. With some RNA viruses, a DNA copy of the viral RNA is infectious. e.g. polioviruses, this permits the preparation of viral genomes, such as those of vaccine strains in high quantities by avoiding the high mutation rate in replication of RNA and its lability.

DNA Virus Replication - Microbiology Book

D. Parvoviruses - The replication of singlestranded parvoviruses is initiated when +ve and -ve stranded DNAfrom different parvovirus particles come together to form adouble stranded DNA molecule from which transcription andreplication takes place.

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