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Nucleic acid and protein synthesis in Staphylococcus aureus.

Nucleic Acid Synthesis: We are equipped with an ABI 8909 Synthesizer.

Peptide Array and Library synthesis: We utilize an IntavisAG SPOT peptide synthesizer to make our peptides for arrays and libraries. Peptides are synthesized on membrane, up to 1536 different peptides per synthesis and cleaved to produce approx. 35 ug of peptide. These can be arrayed onto standard microscope slides up to 6000 spots per slide for peptide binding or activity assays.

Aptamer selection (SELEX): The Center has extensive experience in the techniques employed for in vitro selection (SELEX) experiments.

INTRODUCTIONWith the introduction of methods of rapid nucleic acid sequence determination, synthesis of mixed oligonucleotide probes and computer-assisted analysis of nucleic acid sequences, the use of a single symbol to designate a variety of possible nucleotides at a single position has become widespread over the last few years.

[Duplicating mechanism for peptide chain and nucleic acid synthesis].

nucleic acids & protein synthesis notes b1 - Biology Junction

Modified nucleotidesIn a number of organisms DNA and RNA are modified at certain positions.

The Center for Protein and Nucleic Acid Research, housed in Science Park 3040 Room 290, is a full service facility focusing on Edman Protein sequencing, Peptide Array and Library synthesis, specialty Nucleic Acid synthesis, and Aptamer selection (SELEX). The facility also works collaboratively with the Next Generation Sequencing Core and the Microarray Core to develop and implement novel sequencing and array applications.

Nucleotide Metabolism: Nucleic Acid Synthesis

nucleic acids & protein synthesis notes b1 - Biology …

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Nucleic acid, DNA replication and Protein Synthesis - …

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