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LabBench Activity Plant Pigments and Photosynthesis

Cuvette 5 also showed no change in the percentage of light transmittance because without the presence of chloroplast, the light could not be absorbed to excite the elections and to reduce the DPIP. Without the functions of chloroplast, photosynthesis could not occur because the DPIP would not be reduced and ATP would not be created. Any fluctuations in the data or graph for cuvette 5 could be explained by human or data error.

The function of the DPIP in this experiment is to act as the electron acceptor, replacing the usual NADP found in plants. When the light shines on the active chloroplasts, the electrons are excited, which causes them to jump to a higher energy level thus reducing the DPIP. As the DPIP is reduced, the colour changes from deep blue to colourless, which affects the rate and level of light transmittance when measured by the colorimeter.

In this experiment we were told that we would be measuring the rate of photosynthesis.

Free rate of photosynthesis Essays and Papers - 123HelpMe

Photosynthesis need light to work, so light therefore should speed up the rate of photosynthesis.

This handout includes the Teacher as well as the Student instructions for two labs:
-Paper Chromatography of Photosynthetic Pigments
-Measuring Photosynthetic Rate using DPIPTables for data collection and the questions for data analysis are included on this lab handout.

This handout includes the Teacher as well as the Student instructions for two labs:
-Paper Chromatography of Photosynthetic Pigments
-Measuring Photosynthetic Rate using DPIP

Free rate of photosynthesis papers, essays, and research papers.

I will measure the rate of photosynthesis by counting the number of bubbles that are released by the stem of the pond-weed when the lamp is shone on it from different distances.

Aim: To see if raising the light intensity increases the rate of photosynthesis.

The purpose of this lab is to measure the effect of various conditions of chloroplast on the rate of photosynthesis or percentage of light transmittance. By using unboiled chloroplast in light, unboiled chloroplast in dark, and boiled chloroplast in light, DPIP was placed into each cuvette and a colorimeter was used to measure the rate of light transmittance.

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with the change in rate of photosynthesis


What is the purpose of DPIP in the experiment - …

Prediction : I predict that as I increase the distance between the light source and the Canadian Pondweed (reducing the light intensity), the volume of oxygen produced within the time limit (the measure of the rate of photosynthesis) will decrease.

Transcript of Photosynthesis Lab

Hypothesis:Photosynthesis is a process by which green plants and certain other organisms use the energy of light to convert carbon dioxide and water into the simple sugar glucose....

1 What is the function of DPIP in this experiment DPIP …

Since photosynthesis is the process by which plants, bacteria, and other autotrophic organisms obtain energy to produce sugars, the right conditions and the right environment are necessary in order to carry out this complex process. Based on prior knowledge and information from this lab, cuvette 3 will have the highest percent of light transmittance and the highest rate of photosynthesis.

1 What is the function of DPIP in this experiment DPIP is ..

During the process of photosynthesis, carbon dioxide plus water in the presence of sunlight, enzymes and chlorophyll produce glucose and oxygen as waste product.

visually determine the rate of photosynthesis

Since photosynthesis requires light and functional chloroplast to absorb and produce sugars, without either one, the process is interrupted and cannot function properly. Unboiled chloroplast will have a higher percent of light transmittance than boiled chloroplast because of the impact temperature has on the proteins/enzymes of the chloroplast. As high temperatures, like the boiling point, the heat generated will denature the enzymes/proteins thus reducing its effect on photosynthesis.

Lab: Exploring the Rate of Photosynthesis - The Biology …

It will be very interesting to see how light will influence the rate of photosynthesis in plants and what will happen if they do not get the required light in order to produce starch .

the higher rate of photosynthesis.

Without functional chloroplast to absorb the energy from the light, the electrons will not be bumped to a higher energy level and will not be able to reduce DPIP. Of the two cuvettes with unboiled chloroplast, the cuvette place in front of the light will have a higher percent of light transmittance than the cuvette placed in the dark because with light, energy can be absorbed, DPIP can be reduced, ATP can be created, and photosynthesis can be carried out.

-Measuring Photosynthetic Rate using DPIP

Light is a very important factor in the rate of photosynthesis, in my project I am going to test that plants do need light in order to photosynthesise.

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