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Solid-phase oligonucleotide synthesis - ATDBio

Oh S, Kim BJ, Singh NP, et al: Synthesisand anti-cancer activity of covalent conjugates of artemisinin anda transferrin-receptor targeting peptide. Cancer Lett. 274:33–39.2009. :

Several examples of aspartimide formation during the peptide elongation have been reported, together with the reopening of the aspartimide cycle during the subsequent Fmoc deprotection step, yielding piperidides [64,65,66].

Solid phase peptide synthesis vessel having a medium or coarse porosity fritted glass resin support

Why is solid phase peptide synthesis limited to 70 …

This phenomenon has been attributed to the self-association of the peptide chain by formation of hydrogen bonds [46].

Diketopiperazine formation may be circumvented in Fmoc/tBu SPPS

- by using 2-chlorotrityl chloride resin or other bulky resins such as DHPP-Resin.
- by coupling the appropriate Fmoc-dipeptide in lieu of the penultimate amino acid. In that case a risk of epimerization exists if the C-terminal amino acid of this dipeptide is not Gly or Pro.

In peptide synthesis diketopiperazine formation is a notorious side-reaction at the dipeptide stage and is particularly prone to occur in Fmoc based SPPS because of its mechanism.

Solid-phase peptide synthesis - WikiVisually

A range of cleavage reagents for peptides synthesized on 2-chlorotrityl resin has been described. TFE/AcOH/DCM (1:1:3) has been developed by Barbs [62]. Cleavage is also rapidly attained with 0.5% TFA/DCM as well as with HFIP/DCM (1:4 or 3:7) [63].

For the synthesis of long peptides we recommend to use a protocol in which systematic double coupling is followed by capping.

AB - Using the Fmoc protection strategy of solid phase peptide synthesis, a 10 amino acid peptide was prepared and cyclized in a "head-to-tail" fashion while it was attached to the solid support. Cyclization was accomplished with either BOP or carbodiimide and the peptide was cleaved from the resin and purified using standard protocols.

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solid phase peptide synthesis | resin manufacturers in …


Buchiglas - Solid Phase Peptide Synthesis Equipment (SPPS)

N2 - Using the Fmoc protection strategy of solid phase peptide synthesis, a 10 amino acid peptide was prepared and cyclized in a "head-to-tail" fashion while it was attached to the solid support. Cyclization was accomplished with either BOP or carbodiimide and the peptide was cleaved from the resin and purified using standard protocols.

solid phase peptide synthesis | resin manufacturers in india

With extensive experience in solid and solution phase synthesis, ChemPep provides premium quality custom peptides with the most competitive price.

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Solid Phase Peptide Synthesis & Custom Peptide Synthesis

But, whichever cleavage reagent is preferred, it has to be washed out very carefully after Fmoc removal, the last washing must be neutral. When synthesizing large peptides the duration of Fmoc cleavage should be gradually increased. For safe removal of the deblocking reagent the resin may have to be washed more often.

Solid Phase Peptide Synthesis Research

As mentioned above, the generation and disappearance of Fmoc based chromophors allows the monitoring of the synthesis. Furthermore, samples may be taken to determine the load of Fmoc peptide. The completion of the deprotection reaction may be checked by cleaving samples and analyzing the obtained peptide.

Solid-phase synthesis - Revolvy

Using the Fmoc protection strategy of solid phase peptide synthesis, a 10 amino acid peptide was prepared and cyclized in a "head-to-tail" fashion while it was attached to the solid support. Cyclization was accomplished with either BOP or carbodiimide and the peptide was cleaved from the resin and purified using standard protocols.

Solid Phase Peptide Synthesis - OpenWetWare

A special paragraph will be dedicated to the problems caused by peptide aggregation in the course of the synthesis. This phenomenon is a major cause of trouble as it is difficult to predict, is sequence dependent and no universal solution has been found up to now.

Результаты поиска по "Solid phase organic synthesis"

Soybean lecithin consisting of 90–95%phosphatidylcholine and mPEG-DSPE, andMal-PEG-DSPE were purchased from Avanti Lipid(Alabaster, AL, USA). Cholesterol (CHO) was purchased from ChengduKelong Chemical Company (Chengdu, China). Rhodamine-PE waspurchased from Avanti Lipid. T7 peptide with terminal cysteine(Cys-HAIYPRH) and TAT peptide with terminal cysteine(Cys-AYGRKKRRQRRR) were produced according to the standard solidphase peptide synthesis by Shanghai Jier Bio-Pharmaceutical Co.,Ltd. (Shanghai, China). Cell culture plates were purchased fromWuxi NEST Biotechnology Co., Ltd. (Wuxi, China). Other chemicalsand reagents were of analytical grade and obtainedcommercially.

THE BENEFITS OF TWO PHASES, IN ONE

The T7-PEG-DSPE was synthesizedaccording to literature with a modest modification (,).T7 was conjugated with DSPE-PEG-BTC in 0.01 Misotonic HEPES buffer (pH 7.5) under the reaction conditions of 4 hat 4°C, gentle stirring and 1:2 molar ratio of peptides toDSPE-PEG-BTC. The reaction was traced by TLC untilthe peptide was completely consumed. The mixture was then dialyzedagainst water, and lyophilized. The resulting conjugateDSPE-PEG-T7 was then used to prepare liposomeswithout further purification.

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